Igwe microscope nke fluorescence bụ ụdị mikroskopu anya nke na-eji isi iyi ọkụ dị elu na-amụnye ihe nlere anya wee kpalie fluorochromes dị na nlele ahụ. A na-ejikarị ìhè nke ihe atụ eme ya site na isi iyi nke na-ebunye ọkụ ultraviolet. A na-eji ha eme ihe n'ọtụtụ ebe na ngalaba ndu, ahụike na ụlọ ọrụ mmepụta ihe.
Kedu ka microscope fluorescence si arụ ọrụ?
Igwe microscope nke fluorescence na-eji mercury ma ọ bụ oriọna xenon mepụta ọkụ ultraviolet. Ìhè ahụ na-abata n'ime microscope wee tụọ enyo dichroic - enyo nke na-egosipụta otu ogologo ogologo ogologo ma na-enye ohere ọzọ gafere. Ngosipụta dichroic na-egosipụta ìhè ultraviolet ruo n'ụdị. Ụfọdụ ihe nlere na-enwu n'ụzọ nkịtị n'okpuru ìhè ultraviolet n'ihi na ha nwere ihe ndị na-egbuke egbuke dị ka chlorophyll. Ọ bụrụ na ụdị ihe a ga-elele anaghị apụta n'onwe ya, enwere ike iji ihe eji esiji fluorescent mee ya nke a na-akpọ fluorochromes.
Kedu ihe microscope fluorescence nwere?
1. Ìhè isi iyi
Ebe ọkụ kachasị ewu ewu bụ mercury, xenon, na LED. Mercury na-enye ìhè kachasị mma maka microscope fluorescence. LED na-aghọwanye ewu ewu n'ihi na ha dị ọnụ ala karịa isi mmalite ndị ọzọ na ha na-eri obere ike.
| Oriọna Mercury | Xenon oriọna | Ikanam | |
| Ogologo ogologo nke Ìhè a na-ewepụta | 350-370 nm | 400nm ~ 450nm | 400-700 nm |
| Oge ndụ (awa) | 200-300 | 400-600 | 10000 |
| Uru | 1. Igwe ọkụ dị elu, isi mmalite ọkụ kachasị. 2. Ọ na-ebupụta ultraviolet siri ike na-acha anụnụ anụnụ-violet iji kpalie ụdị fluorophores niile. 3. Ihe onyonyo na-egbuke egbuke ma mara mma. | 1. The spectral ike siri ike. 2. Ike spectral siri ike na infrared na etiti infrared. | 1. Ọ dị mfe iji dochie anya. 2. Ọ dịghị mkpa ikpo ọkụ. 3. Enwere ike ịgbanwuo ma gbanyụọ ozugbo. 4. Njikwa ike isi iyi ọkụ. |
| Mwepu | 1. Ogologo oge ndụ. 2. Ogologo oge ikpo ọkụ. | 1. Pụrụ iche obere voltaji DC ike igbe chọrọ maka oke okpomọkụ. 2. Dị ọnụ karịa oriọna Mercury | 1. UV ìhè adịghị ike karịa mercury oriọna. 2.Ọ bụla wavelength nke ìhè chọrọ iche iche LED n'ihi na warara bandwit ha emit. |
Mgbakwụnye Mercury Fluorescent
Mgbakwụnye LED Fluorescent
2. Ihe nzacha mkpali
Ihe nzacha mkpali dị mkpa maka ịrụ ọrụ nke microscope fluorescence. Ọ na-agafe ìhè nke ogologo ebili mmiri dị mkpụmkpụ, nke ụcha fluorescent nwere ike ịmịkọrọ. Ọzọkwa, ọ na-egbochi isi iyi ndị ọzọ nke ìhè na-akpali akpali.
Ihe nzacha mkpali (B,G,U,V)
3. Dichroic mirror
Ngosipụta dichroic bụ ụdị nzacha anya nke na-egosipụta ìhè n'ogo ụfọdụ n'ogologo ma na-ebufe ndị ọzọ. A na-eji ya na microscopes fluorescence iji kewapụ mkpali na ikuku ikuku.
4. Emission Filter
Ihe nzacha na-apụ apụ na-agafe naanị ogologo ebili mmiri nke fluorophore na-ewepụta ma na-egbochi ọkụ niile na-achọghị n'èzí ìgwè a - karịsịa ogologo oge mkpali.
5. Fluorescent agbaji
Ihe e ji esiji na-egbuke egbuke bụ ogige organic nke nwere ihe na-egbuke egbuke, nke ha nwere ike ịmepụta ihe oyiyi fluorescent site n'iwepụta ìhè akwụkwọ ndụ akwụkwọ ndụ dị iche iche a na-ahụ anya mgbe ọkụ ultraviolet na-egbuke egbuke masịrị ya. Ihe eji esiji fluorescent a na-ejikarị eme ihe bụ; DAPI (49,6-diamidino-2-phenylindole), oroma acridine, auramine-rhodamine, Alexa Fluors, ma ọ bụ DyLight 488.
Kedu ụdị microscopes fluorescence ole?
1. Microscope Epifluorescence kwụ ọtọ:
Ọ bụ ụdị microscope nke fluorescence a na-ahụkarị. A na-eme mkpali nke fluorophore na nchọpụta nke fluorescence site n'otu ụzọ ọkụ ahụ (ya bụ site na ebumnobi). Ihe ka ọtụtụ n'ime microscopes fluorescence, karịsịa ndị ejiri na sayensị ndụ, bụ nke epifluorescence imewe.
BS-2081F nyocha nke Fluorescent Biological Microscope
2. Microscope Confocal Fluorescence:
Confocal Fluorescence Microscope: Ụdị microscope nke fluorescence na-ejikọta nyocha laser na ìhè fluorescent iji mepụta ihe oyiyi. Enwere ike iji ya n'ọtụtụ ngwa dị iche iche, dị ka ịmụ mkpụrụ ndụ na anụ ahụ, ịchọpụta protein na ihe ndị ọzọ dị n'ime sel, na ịlele nha nke ihe.
BS-2081F nyocha nke Fluorescent Biological Microscope
3. Microscope nke fluorescence tụgharịrị
Ụdị ìhè microscope na condenser dị n'elu, chere ihu ala. Igwe ọkụ ga-adịrịrị na ogo 90 n'ihe gbasara elu ihe nlere anya a na-enyocha.
BS-2095F Mikroskopu nke Fluorescent tụgharịrị
Kedu uru na ọghọm dị na microscopes fluorescence?
Uru:
- Na-enyere aka ileba anya nke mkpụrụ ndụ n'ime ihe ndị dị ndụ na-emebighị ha
- Na-enye ihe onyonyo dị elu nwere agba ziri ezi
- Na-enye ohere maka ọmụmụ nke usoro ndụ na sel
- A ga-eji chọpụta ụdị molekul dị iche iche dị n'ime sel, dịka protein ma ọ bụ acid nucleic.
Ihe ọghọm:
-Ọ na-enye ohere ịhụ ụfọdụ ihe owuwu dị n'ime cell nke ejiri eriri fluorescent akara.
-Ịchacha foto n'ihi mkpali eletrọn n'oge usoro nke fluorescence nwere ike imetụta ụmụ irighiri ihe na-arụ ọrụ nke agba agba fluorescent. N'ihi ya, ihe na-esiji ihe na-emeghachi omume nwere ike tufuo akụrụngwa kemịkalụ ha nke ike ikuku fluorescence.
-Mkpụrụ ndụ ndị ahụ na-enwe ike ịnweta mmetụta fototoxic mgbe ha na-eji ihe na-acha fluorescent mee ihe, dị ka ụmụ irighiri fluorophore na-amịkọrọ foton ike dị elu site na ìhè nke dị mkpụmkpụ.
Kedu ihe bụ ngwa nke microscope fluorescence?
A na-eji microscopes fluorescence eme ihe n'ọtụtụ ebe nyocha na ngwa gụnyere biochemistry, cell bayoloji, microbiology, immunology, na ọgwụ.
1. N'ihe gbasara bayoloji, microscope fluorescence na-enyere aka ịmata nke ọma na nkọwa zuru ezu nke cellular na submicroscopic cellular components na ihe omume site n'enyemaka nke akara ngosi fluorescent.
2. Na ngalaba ahụike, microscope fluorescence nwere ike iji fluorescent reagents chọpụta ọnụnọ na nkesa nke nje bacteria na nje, ma ọ bụ nyere aka n'ịkpọ aha ịwa ahụ iji kwado ịwa ahụ.
3. N'ihe gbasara mineralogy, a na-ejikarị microscope fluorescence na-amụ ihe ndị nwere ihe ndị na-egbuke egbuke na-eme onwe ha, dị ka asphalt, petroleum, coal, graphene oxide na mineral ndị ọzọ.
4. Na sayensị ihe, enwere ike iji microscope fluorescence na ụlọ ọrụ textile ma ọ bụ ụlọ ọrụ akwụkwọ iji nyochaa ihe ndị dabeere na fiber.
Oge nzipu: Mar-14-2023