BCF297 Laser Scanning Confocal Microscope
BCF297 bụ microscope confocal na-enyocha laser ọhụrụ ewepụtara, nke nwere ike nweta nleba anya dị elu na nyocha ziri ezi. Enwere ike iji ya mee ihe na morphology, physiology, immunology, genetics na mpaghara ndị ọzọ. Ọ bụ ezigbo onye mmekọ maka nyocha nke biomedical.
Atụmatụ
1. Oke mgbama na mkpọtụ ruru.
Ụzọ ngwa anya confocal imaging arụmọrụ dị elu nwere ike inye onyonyo ncha ọkụ nwere oke mgbama na mkpọtụ oke ọbụlagodi n'okpuru ncha ọkụ adịghị ike.
2. Ihe oyiyi mara mma.
Nnukwu ụdịdị dị iche iche, oghere oghere ọnụọgụ dị elu, zuru oke maka agbapụ ụdị confocal dị iche iche.
3. Dị mfe iji.
Igwe ọkụ eletrik zuru oke, nhazi kachasị mma nke mmekọrịta mmadụ na kọmpụta, na-enye gị ohere ịrụ ọrụ n'ụzọ dị mfe n'oge ịgba égbè.
4. Niile moto Control System.
The Z-axis nke BCF297 laser confocal microscope nakweere ngwaọrụ moto, nke nwere ike ịhazigharị elu Z-axis ngwa ngwa dịka onyonyo a na-ahụ anya. AF One-bọtịnụ autofocus, na-ewepụ mkpa maka nhazi nhazi nke ọma na imeziwanye arụmọrụ ọrụ.
Bọtịnụ njikwa agbakwunyere n'akụkụ abụọ nke ahụ nwere ike ịghọta ngwa ngwa ma ọ bụ ntụgharị nke condenser, nchapụta, oghere ebumnuche, ihe nkiri attenuation na-atụgharị, na ntụgharị fluorescence, nke na-eme ka ịdị mma nke ọrụ dịkwuo mma.
5. Optics kachasị elu maka onyonyo ngbanwe.
(1) Ọdịdị pinhole onyonyo pụrụ iche: wedata nnyonye anya nke nchụpụ akụrụngwa na-ebute ma melite ogo mgbama na mkpọtụ na mkpebi axial nke onyonyo a.
(2) Ngalaba nchọpụta njikwa: ngalaba nchọpụta nwere mmetụta dị elu (kachasị QE≥45% @ 500nm), nke nwere ike mechaa eserese confocal nke ọtụtụ agba na-akpaghị aka nke ọma na ngwa ngwa.
6. Abụọ tent nke nhọrọ lenses ebumnobi.
BCF297 laser scanning confocal microscope nwere oghere abụọ nke nhọrọ ebumnuche nhọrọ, apochromatic (2X-100X) na super-apochromatic (10X-100X), nke nwere nnukwu mkpuchi ịdị elu, dabara maka nyocha nyocha dị elu na mwepu onyonyo onyonyo. Nnukwu oghere ọnụọgụ na-eme ka mkpebi dịkwuo mma.
Ebumnuche APO enweghị ngwụcha
Ebumnuche Super APO enweghị ngwụcha
7. Software pụrụ iche maka confocal laser.
Ngwanrọ maka microscope confocal a juputara na njiri dị ike. BCF297 na-akwado otu ọwa ma ọ bụ ọtụtụ ọwa 2D imaging (XY), onyonyo 3D (XYZ), onyonyo 4D (XYZT) na nyocha ọtụtụ saịtị. Enwere ike ịme eserese, fotobleaching na photostimulation na onye ọrụ akọwapụtara ROI (mpaghara Mmasị), Z-Stack imaging, nnukwu ihe ntanetị ihe oyiyi, nhazi ọkwa, nhazi nzacha, ndekọ data, wdg.
Nkọwapụta
| Ihe | Nkọwapụta | BCF297 | |
| Isi iyi ọkụ laser | Laser | 405nm/50mW, 488nm/50mW, 561nm/50mW, 640nm/40mW | ● |
| Njikwa laser | Modulation ozugbo na acousto-optic modulation, ihe ọkụ ọkụ laser niile na ike nwere ike gbanwee ozugbo, ma nwee ike ịbanye na mpụga steeti ozugbo ma ọ bụrụ na ejighị ya obere oge. | ● | |
| Nyochaa modul | Ngalaba nyocha | Dual-axis XY elu-ọsọ nyocha ngwa anya galvanometer | ● |
| Ubi anya 14mm × 14mm (≥ 19) | ● | ||
| pikselụ nyocha 512×512～4096×4096 | ● | ||
| Oge Pixel 0.5μs ~ 8μs | ● | ||
| Ọsọ nyocha ọkọlọtọ: 1fps (512×512, 2μs) Ọsọ nyocha ngwa ngwa: 3fps (512×512, 0.5μs) | ● | ||
| Nyochaa mbugharị: 1-32X | ● | ||
| Pinhole | Ф30/40/50μm okirikiri pinhole, nke dị n'etiti dichroic beam splitter na scanning galvanometer, iji hụ na fluorescence collection arụmọrụ nke usoro na-mgbe niile na-edobe na 100% | ● | |
| Dichroic mirrors na nzacha | Ọwa anọ dichroic beam splitter: 405/488/561/640nm | ● | |
| 6-ọnọdụ moto filter wheel na-abịa ọkọlọtọ na anọ: 445nm/40, 530nm/43, 607nm/36 na 685nm/40 | ● | ||
| Ngalaba nchọpụta | MA PMT, QE ≥ 25%@500nm, 20%@600nm (ọnụọgụ PMT 1) | ● | |
| GaAsP PMT, QE ≥ 45%@500nm, 40%@600nm | ○ | ||
| Ngalaba nchọpụta DIC | Site na ọrụ onyonyo nnyonye anya dị iche, ọ nwere ike ghọta onyonyo "DIC-fluorescence". | ● | |
| Mikroskopu tụgharịrị n'ọkwa nyocha | Sistemụ anya | Sistemụ ngwa anya emeziri Infinity Chromatic Aberration | ● |
| Isi nleba anya | 20°-45° mgbanwe mgbanwe, oyiyi tụgharịa, infinity hinged binocular observation tube, interpupillary anya mgbanwe nso: 50 ~ 76mm | ● | |
| Anya anya | Elu anya n'obosara mbara ubi atụmatụ eyepiece PL10X/22mm, mgbanwe diopter, micrometer | ● | |
| oghere ebumnobi | Atụmatụ Infinity Apochromat Ebumnuche 4x, 10x, 20x, 40x, 60x, 100x | ● | |
| Atụmatụ Infinity Super Apochromat ebumnobi 10x, 20x, 40x, 60x, 100x | ○ | ||
| Igwe microscope | Ọnọdụ aka dị ala na-enweghị isi na micro coaxial electric mbanye na-elekwasị anya usoro, Z-axis ọrịa strok 10.5mm, nkenke 1um; n'ihu nnukwu-size LCD mmetụ ngosi panel, integrated electric akara bọtịnụ na ahu, nke nwere ike na-aghọta condenser, nchapụta, ebumnobi oghere, ngwa anya n'ọdụ ụgbọ mmiri, wdg Quick mgba ọkụ n'etiti ọrụ. Ọdụ ụgbọ mmiri glazing wuru n'ime ya, nke dị n'akụkụ aka ekpe nke fuselage, nkewa nkewa 100:0, 0:100; ọdụ ụgbọ mmiri aka ekpe arụnyere n'ime ya, oke nkewa 0:100, 50:50, 100:0; single-layer/ double-layer optical path optional , na-enye ohere maka mgbasawanye usoro, na otu igodo ngbanwe nke modul ụzọ anya nke oyi akwa ọ bụla dị ka mkpa. Ya na Fluorescent Visor, Ihe Ntụgharị Moto 6-Position (ya na oghere DIC) yana ngwa nkwụnye CTV Port glazed | ● | |
| Ogbo | Akwụkwọ ntuziaka n'ibu ikpo okwu, table size 300mm (X) × 240mm (Y), na-akpụ akpụ nso 135mm (X) × 85mm (Y) Eletriki ogbo, okpokoro size ọ bụghị ihe na-erughị 260mm (X) × 153mm (Y), na-akpụ akpụ nso 110mm (X) × 75mm (Y), na onwe-arụ ọrụ aka; kacha ọsọ 3mm/S, kwugharịa n'ọnọdu ziri ezi ± 1um; Ejiri ya na efere petri 35mm, nlele ngalaba | ○ | |
| Condenser | Igwe ọkụ nwere oghere 7, NA 0.55, WD 27mm; Oghere 3 maka φ30mm (ọdịiche nke oge), oghere 4 maka φ38mm (DIC); na-akwado ubi na-egbuke egbuke, ọdịiche nke oge, nleba anya DIC (gụnyere ntọala polarizer) | ● | |
| Sistemụ ọkụ ọkụ fluorescent | 8-oghere fluorescent usoro ntụgharị, usoro ahụ nwere ike ikpebi ọnọdụ nke turntable na-etinye n'elu elu na nke ala nke rack; ọ nwekwara ọrụ mkpuchi ọkụ eletrik, nke nwere ike igbochi isi iyi ọkụ fluorescent ozugbo, yana otu enyo ihe nzacha fluorescent: B/G/UV, wdg. | ● | |
| Akụrụngwa na-arụ ọrụ
| Ịnyocha onyonyo | Nnweta onyonyo na sistemu njikwa akpaaka, njikwa ọkụ eletrik zuru oke nke ngbanwe ụzọ anya; ntughari nke onwe nke igwefoto igwefoto na ihe nleba anya; nlele zuru oke na onyonyo nyocha ROI; otu agba ma ọ bụ multi-agba 2D imaging (XY), 3D imaging (XYZ), 4D imaging (XYZT) na ọtụtụ saịtị nyocha. | ● |
| Nyocha nhazi | Multi-color fluorescence collocalization nhazi, fluorescence na nnyonye anya dị iche iche (DIC) oyiyi superposition; nhazi na ịgbakwunye akpịrịkpa; nhazi nhazi; Nyocha nhazi Z-Stack na nnukwu ihe nkedo ihe oyiyi | ● | |
| Njikwa data | Njikọ ngwaike dị iche iche bụ nhọrọ, enwere ike ịtọ ụzọ nchekwa onyonyo akpaka, ọtụtụ ụdị mmepụta ihe onyonyo, yana ihe nleba anya nwere ike ịchekwa ozugbo. | ● | |
Mara: ●Standard ekike, ○ Nhọrọ
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